Centrifuge Calculator
Created by
Eon CalculatorWhat is a Centrifuge?
A centrifuge is a machine that spins really fast. This machine is majorly used in science, medicine and few other industries to separate different molecules of a solution mixture. For example, you can separate blood into plasma and cells.
When a centrifuge spins, it creates a force that pushes heavy materials outward. Lighter parts stay closer to the center. This spinning force is called centrifugal force. Centrifuges are used in many fields like hospitals, research labs, food and drink industry.
What is Centrifugal Force?
Relative Centrifugal Force
What is Revolutions Per Minute
Convert between RPM and RCF
How to convert RCF to RPM
When your centrifuge allows you to enter only RPM values and your protocol specifies only RCF, you can use this formula using the rotor radius.
How to convert RPM to RCF
For example: A centrifuge at 5000 RPM and a 12 cm radius: RCF = 11.18 × 12 × (5)^2 = 11.18 × 12 × 25 = 3,345 xg.
| Use Case | Typical RCF (×g) | RPM (varies with radius) |
|---|---|---|
Blood plasma separation | 1,200–3,000 ×g | ~2,500–4,000 RPM |
Cell pelleting | 5,000–8,000 ×g | ~5,000–10,000 RPM |
Bacterial pellet isolation | 6,000–12,000 ×g | ~7,000–13,000 RPM |
DNA extraction | 10,000–14,000 ×g | ~10,000–15,000 RPM |
RNA extraction | 12,000–16,000 ×g | ~12,000–16,500 RPM |
Virus concentration | 100,000–200,000 ×g | ~40,000–60,000 RPM |
Protein purification | 20,000–50,000 ×g | ~20,000–35,000 RPM |
Organelle isolation | 600–2,000 ×g | ~1,000–3,500 RPM |
Organelle isolation | 10,000–15,000 ×g | ~9,000–14,000 RPM |
Yeast cell pelleting | 3,000–5,000 ×g | ~3,500–6,000 RPM |
Exosome isolation | 100,000 ×g | ~40,000–55,000 RPM |
Table 1 shows common use cases with their RCF and RPM values.
How to measure rotor size
Nominal radius:
Effective radius:
Maximum radius:
Centrifuge Calculator
Input one and get the other
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